IVF Treatment in India, Test Tube Baby Services in Delhi
The technique of IVF consists of bringing about the fertilization of the oocyte and the spermatozoa in the laboratory instead of
in the oman!s fallopian tube" IVF involves induction of ovulation in order to obtain multiple oocytes, thus ma#ing available
more embryos ith hich higher pregnancy rates can be achieved" Serial determination of plasma estradiol levels and daily
monitoring of ovarian follicular groth by ultrasonography ould indicate the response to ovarian stimulation" $t the
appropriate moment of follicular groth, the follicles are aspirated to obtain the oocytes" The oocytes are mi%ed ith
appropriately capacitated spermatozoa from the husband &or the donor, if the medical condition indicates the use of donor
sperm' and is #ept in an incubator for fertilization hich is observed microscopically after () to (* hours" +mbryos are
transferred into the uterine cavity beteen days , to ) after oocyte aspiration" If implantation ensues, pregnancy can be
confirmed by (- to () days after embryo transfer by determining the presence of h./ in a blood or urine sample"
.ommon indications0
•Bloc#ed or absent fallopian tubes1 Fertilisation of the egg occurs on its 2ourney through the fallopian tube so if the tubes
are bloc#ed or absent, this process cannot occur"
•+ndometriosis 1 This is hen the lining of the omb can be found covering other pelvic structures such as the ovaries,
pelvic ligaments, peritoneum &lining of the pelvis', boel, and bladder" This causes irritation and inflammation hich can
lead to scarring" It is also associated ith infertility, although the reasons for this are not fully understood"
•Irregular ovulation 1 For ovulation to occur, part of the brain prompts the pituitary gland to secrete hormones for the
ovaries to ripen eggs during the menstrual cycle" If the menstrual cycle does not occur or is irregular, ovulation may also
be irregular or absent"
•3o sperm count or motility 1 If there are not enough sperm released in the semen or the sperm that are released are
not mortile enough to ma#e it to the oman!s reproductive system, conception is not possible"
•4ne%plained Infertility 1 In some cases, after all investigations have been completed, no medical reason is found to
e%plain the inability to conceive
Steps in IVF Treatment
(" Stimulation of the 5varies 1 $ series of in2ections of a hormone called FS6 are given to stimulate the ovaries to produce
more than the usual one egg per cycle"
," 7revention of 7remature 5vulation 1 This is achieved by shutting don communication beteen the brain and the ovaries
to gain control of the ovulation day so that the eggs are not released before they can be collected"
8"Triggering 5vulation 1 $n in2ection is given to trigger the ovulation process"
-".ollection 1 The eggs and sperm are collected to begin fertilization" &+gg collection done under short general anesthesia
transvaginally under ultrasound guidance'
9".ulturing 1 The eggs are sorted in the laboratory" The mature ones are selected and placed ith the sperm in a plastic
container" If more embryos are created than are required for transfer, the strong healthy ones may be put in cryo:storage
&frozen' for transfer at a later date if required"
)"Transfer 1 The embryos are transferred through the cervi% and into the uterus" The success of IVF dependents upon a
number of factors including a oman!s age, the cause of infertility, and the quality of sperm"
$SSIST+D 6$T.6I;/
$fter an egg is fertilized in the laboratory, the cells begin to divide" During these initial stages of development, the embryo is
contained in a layer of proteins #non as the zona pellicuda" In order to successfully implant into the uterine lining, an
embryo has to hatch out of the zona pellucida and attach to the alls of the uterus"
$ssisted hatching is a neer lab technique that as developed hen fertility e%perts observed that embryos ith a thin zona
pellucida had a higher rate of implantation during IVF" <ith assisted hatching, an embryologist uses micromanipulation under
a microscope to create a small hole in the zona pellucida"
Indications
$ssisted hatching is helpful for couples ith a poor prognosis hose embryos are thought to lac# sufficient energy to
complete the hatching process"
$ssisted hatching may be indicated for omen ith0
•$dvanced maternal age &older than 8*'
•To or more failed IVF cycles
•Thic# zona &covering' of embryo
Dr Surveen Ghumman Sindhu
7hone ;o" 0 =>(: >*(?-@9-@)
+mail Id 0 surveen(,Agmail"com
$ddress0 B 9(@ ;e Friends .olony, ;e Delhi, India ((??,9
www.ivf-india.in