Bulk Peptides

Bulk Peptides
Posted on December 30, 2013 by Maxim Peptide
As researchers improve the methods used to produce synthetic
peptides, it is becoming easier to create larger batches of peptides
at once. This is performed by creating chemical bonds that will
mimic the reactions created by an animal’s body to produce a
similar sequence of amino acids that would create a hormone or
chemical reaction within the body. n some cases, the bonding
sequence of the amino acids are altered when producing synthetic
peptides because it can alter the reaction of the peptide or increase
the half!life of the peptide once it is applied to an animal’s blood
stream. "reating bulk peptides can pose difficulties for
manufacturers as the process can be somewhat unpredictable. The
proper methodology for creating bulk batches of peptides vary
based on the nature of the chemical. The effectiveness of the
methods used to create the necessary chemical bonds to create
amino acid chains that produce peptides is still under review, and it
is not uncommon for different companies to utili#e different methods
in order to create these chains. The key is utili#ing methodologies that are determining the most appropriate
method for creating bulk peptides is the process that ensures the highest level of consistency in the final batch of
chemicals.
Reconstituting Bulk Peptide Batches
$very peptide will have different instructions, based on how it was formed and what chemicals are included in the
bonds, but there are a few basic guidelines that should be followed to ensure that a viable solution is created.
• f a peptide is designed to be used with a specific liquid, these instructions should be included with the powder.
f the instructions do not make any particular specifications, bacteriostatic water can be used to re!constitute the
powder. Almost all peptides can be safely combined with this substance% this is a very cost effective solution for
researchers that are working with bulk si#ed batches.
• There is an e&ception to this rule. '( or any variations in this peptide should be reconstituted with acetic acid
solutions. These typically cannot be combines with any other type of liquid.
• The amount of liquid or peptide powder should be carefully measured before being inserted into a vial for
application into a live animal test sub)ect. $very peptide should contain a set amount of applications in the
bottle, and this cannot be varied, due to the strength of the solution that was created. n general, the amount of
peptide and research liquid that is added should always equal the same weight, which will be specified on the
container. f there is no specification, be sure to contact your bulk peptide supplier to determine the proper
instructions for this step. n most cases a bulk peptide order will also include syringes that are intended for use
in animal testing in research facilities, to ensure accuracy with this part of the process.
The strength of an application can be altered as necessary by adding more or less of the peptide in a given
solution, so long as the total weight of the solution remains the same within the syringe. This is a common method
that is used to determine what application si#e is ideal to elicit the type of response that researchers are hoping to
achieve. t is also helpful for determining what application si#es could be dangerous to in)ect into animal tissue
because it elicits side!effects.
Creating Long Term Shipping Schedules
"ompanies that produce bulk sized amounts of peptides often create partnerships with research facilities* to
continue providing the supplies necessary for long!term research pro)ects.
• Those that have a set schedule for ongoing trials can ensure that multiple shipments of peptides that coincide
with this schedule.
• +hipments of different types of peptides can be scheduled to better ensure that these materials can be
compared in animal tissues. This is essential for any research that is working to determine what type of peptide
is ideal for the type of reaction that is being triggered.
Bulk peptide suppliers can help researchers determine the best timing for receiving new shipments of product.
+tudies that will be ongoing for several years will need to work with multiple shipments of peptides to ensure that
the peptides are fresh and stable enough to work effectively. ,ost peptides can be kept in cold storage for up to two
years if they are kept sealed, moisture!free and only constituted when it comes time to apply them to animal test
sub)ects. The necessary information to make these decisions should be available on the sale page for the given
peptide, but a bulk peptide supplier can always be contacted to answer any additional questions.
Seeking out a Source for Bulk Peptides
The consistency of product that is made available from a company will help researchers determine whether or not it
is appropriate to utili#e these wares for their e&periments.
• Bulk peptide companies are often willing to share which methodologies they use to create peptides to
researchers. This helps people increase the depth of their research by comparing the best methods for creating
accurate or reliable peptides.
• Bulk peptide producers will also typically share their accuracy rates for producing peptides. This will help
researchers determine if these peptides are reliable enough to create reactions that can be used as a definitive
benchmark for peptide reactions and the development of future research that will depend on the predictability of
these reactions.
• n addition to noting the purity level of a peptide that is listed with a sample available for purchase, it is important
to note which method was used to determine this purity ranking. f this information is not available on a sales
page, the company should be forthcoming with this information.
• The only way to fully determine the purity of a sample is to perform a full amino acid analysis. -hile it is
impossible to do this on a full bulk sample, there is a range of testing procedures* including mass spec, .P/"
and +0+ page* that can provide an indication of what is included in this sample. .owever, the more chemicals
and mi&tures included in a component, the less accurate methods such as .P/" will be in determining purity,
which can influence the safety of using these samples in a research setting.
A great deal of consideration must be given to the storage of peptides after they have been purchased. They must
be kept in containers that will ensure that other particles, particularly moisture inducing elements are not able to
interact with the peptide. This will damage the structures of the amino acids and can cause a breakdown in their
chemical structure. +imilarly, it is also essential that peptides are kept at a consistent temperature to avoid damage
during transit and before they are applied to animal tissues. 1nce a peptide is received by a research facility similar
care must be given to ensure that peptides are not damaged or begin breaking down before they have been
applied to the animal tissues. nstructions for how peptides should be reconstituted and applied should be included
with the vials so that a consistent procedure can be followed during this phase of an e&periment. Typically, peptides
should only be reconstituted when they are to be applied to the animal test sub)ect, though some are able to remain
in their constituted form for up to two weeks without the fear that they will begin showing signs of instability.
Resource Box2
http233en.wikipedia.org3wiki3Peptide4sequence