Research Scientist or College Professor
Content
Resume PHILIP F. JEMILOHUN, Ph.D. 4000 Gillionville Road #40 Albany, GA 31721 Phone: (229) 432-9023 Cell: (229) 288-6070 E-mail: [email protected] ________________________________________________________________________________ __ Summary To obtain a scientist position in an industry/academic laboratory where my educa tion, experience and ability/skill in microbiology, molecular biology, and immun ology will be utilized and thereby contribute to the overall growth and success of the industry/establishment. Education Postdoctoral Research Fellow. 1993. Meharry Medical College. Nashville, Tenness ee. Ph.D. Biology (Mol. Biol.) 1989. Clark Atlanta University. Atlanta, Georgia. M.S. Microbiology. 1981. East Tennessee State University. Johnson City, Tennessee. B.S. Medical Technology. 1978. California State University. Los Angeles, Califo rnia. Research Experience Currently, I am investigating pathogenesis of human and bovine trichomoniasis ca used by flagellated protozoans, Trichomonas vaginalis and Tritrichomonas foetus respectively with initial focus on flagellar adhesins and antigens. * Have developed protocols for isolating and purifying the flagella using physic al/chemical homogenization and series of density centrifugations techniques. * Have characterized the flagella polypeptides by SDS-PAGE, immunoblot, immunofl uorescence and ELISA assays. * Novel flagellar and cellular adhesion molecules have been detected by function al adhesion and blot overlay assays. * Extensive experience in tissue culture, molecular and microbiological techniqu es. 1989 -1993 Postdoctoral Fellow. Meharry Medical College, Nashville, Tennessee. Division of Biomedical Sciences. Construction and screening of cDNA library of T rypanosome cruzi in gt11. Purification of antigens. Expression of the surface an tigens of T. cruzi in gt11. Amplification and screening recombinant gt11 by PCR methodology. Southern and Northern analyses of genes and Western blot of surfac e protein antigens of T. cruzi and DNA sequencing. Identification and analysis o f developmentally expressed/regulated surface proteins of T. cruzi. 1984 -1989 Graduate Assistant, Clark Atlanta University, Atlanta, Georgia Investigation of DNA-membrane interaction (adhesion) using plasmid R6K as a mode l. Research included isolation, purification and restriction digest analysis of the plasmid DNA; hybridization, gel electrophoresis and plasmid DNA-membrane bi nding analysis; transformation and end labeling with radioactive ATP; isolation and purification of cell membrane. 1982 -1983 Research Assistant, Centers for Disease Control, Atlanta, Georgia. Investigation of Campylobacter jejunum by direct Immuno-fluorescent antibody ass ay. 1978 -1981 Graduate Student, East Tennessee State University, Johnson City, Tenn essee. My thesis research focused on aStimulation of Pseudomonas aeruginosa by C
andida albicans and Torulopsis glabrata in the presence of gentamicina. Research Instrumentation and Computer Utilization Skills a Utilized RT-PCR, construction of cDNA library, RNA/DNA isolation and Western/s outhern/northern blot techniques. ELISA assay, adhesion assay, blot overlay assa ys. a Use bio-markers in monitoring and interpreting tissue changes a High Performance Liquid Chromatography (HPLC) a" For separation of biological and chemical samples for bioactivity purposes. Philip F. Jemilohun 2 a Microscopy (fluorescence and light) for identification/recognition of sub-cell ular parts a High speed centrifugations for separation/purification of biomolecules and org anelles. Statistical Analysis, excel, SPSS, Word Processing, Microsoft Word and Word Perf ect Visual Presentation, Paint, Power Point, PhotoShop Teaching Experience 2007 - 2009. Associate Professor. Albany State University, Department of Natural Sciences, Albany GA. Teaching assignment included Microbiology, Genetics and sp ecial topic in ecology (Microbial Ecology) at graduate level and Immunology, Gen eral Biology, Study Skill, Research/Independence study and Introductory Biology at undergraduate level. I participated in mentoring HBCU-UP/STEM students in res earch and also supervised senior research projects. The findings from some of th e student projects have been presented at many national conferences. 2002 - 2006. Associate Professor. Biology Department, University of Arkansas, P ine Bluff Arkansas. Teaching assignment included Cell Biology, Microbiology, Par asitology and General Biology. 2000 - 2002 Virginia State University, Petersburg. Virginia. I taught Microbiolo gy, Immunology, Virology, Cell Physiology, Molecular Biology and Introductory Bi ology courses. 1993 - 2000 Assistant/Associate Professor. Jackson State University, Jackson. Mi ssissippi. I taught Microbiology and Molecular Cell Biology at both graduate and undergraduate levels, Independent study and General Biology/Introductory Biolog y courses for biology majors and non-majors. I developed a Molecular Biology cou rse for undergraduate students. Faculty Research Mentor I supervised research projects of three undergraduate students (two on MARC, and one on ONR programs). I also supervised three master degree theses and I was a member of dissertation committee. I was also a faculty research mentor and an i nstructor for Bridges to the Baccalaureate Degree Program (BBDP), Jackson State University/Hinds Community College. Minority students from Hinds Community Colle ge performed research in my laboratory during academic year and summer. Their re search studies have been presented at various symposia such as at University of Memphis, Tennessee in summer 1996, Georgian State University, Atlanta, Georgia i n summer 1997 and University of Southern Alabama, Mobile, AL in summer 1999. Professional Affiliation and Awards American Society for Microbiology Beta Kappa Chi Scientific Honor Society Minority Biomedical Research Service (MBRS) award for doctoral program National Research Service Award for postdoctoral training 1990-1992 Research Grants 2008. Faculty Development Award for $10,000.
2003-2006. NIH/AREA grant: aFlagellar antigens from Trichomonas vaginalisa was f unded for $100,000. 1995. Faculty Research Award by Mississippi Urban Research Center (MURC) for $10 ,000 1994. Awarded aseed funda for $4,500. Professional Development Various courses, seminars, workshops and conferences supplement my extensive exp eriences in teaching, services, and research. a DNA micro-array workshop. Morehouse College Atlanta Ga. July 2005 a Quality Education for Minorities (QEM) Network/NSF proposal workshop. Janua ry 2005. a Served as a judge at many regional and state science fairs. a Attended Y2K Research/Teaching Symposium. Washington University, St. Louis M O. June 2000 Philip F. Jemilohun 3 Publications C Philip Jemilohun. Analysis of adherent and non-adherent Trichomonas vaginalis and identification of potential novel adhesins. Manuscript in preparation. a Philip Jemilohun and Tedra Thomas. Immunogenicity and adhesive property of fl agella from Trichomonas vaginalis. J. Parasitology. Manuscript in preparation. C Jemilohun, P.F. Garnett, T. and Burgess, D. E. 2005. Isolation, partial purifi cation, and immunogenicity of flagella from Tritrichomonas foetus. J. Eukaryotic Microbiology, 52:245-249. a Jemilohun, P.F. 1998. Isolation and Characterization of Flagella from Trichomo nas vaginalis. Parasitology Research. 84: 800-805. a Jemilohun, P. F.; Clark, C. W. and Archibold, E. R. 1996. Characterization of Bacterial Cell Membrane Attachment Sites of Plasmid R6K. Biochem. and Biophys. Res. Commun. 221: 186-192. a Jemilohun, P. 1991. Developmentally Regulated Surface Polypeptides of Invasi ve forms of Trypanosoma cruzi. Tropical Diseases. Meharry Medical College Train ing Program Newsletter. Abstracts & Poster Presentations a Jemilohun, P. F. Analysis of adherent and nonadherent Trichomonas vaginalis c ells and identification of potential novel adhesins. UAPB Faculty and Students R esearch Forum. March, 2006. a Jemilohun, P.F. Characterization of flagella from Tritrichomonas foetus. Abs tract D-141. 101 Annual General Meeting, American Society for Microbiology 2001, Orlando, Florida. May 2001. a Layfield, N., Luckett, J. and Jemilohun, PF. 1999. Flagellar Antigens of Trit richomonas foetus. National Minority Research Symposium. Phoenix. November 10-13 . a Hall, A., Wright, K. and Jemilohun, P.F. Characterization of flagella from T ritrichomonas foetus. National Minority Research Symposium. New York City. No vember 1998. a Barber, L.P. and Jemilohun, P.F. SDS-PAGE Analysis of flagella from Trichomo nas vaginalis and Tritrichomonas foetus. Mississippi Academy of Science Annual Meeting, 1998. a Mary Green, Chelsea Spann, Benjamin Torrey, Toshma Thomas-Carter and Philip J emilohun. SDS-PAGE Analysis of Flagella from Tritrichomonas foetus. Second Annua l Bridge Research Conference, Georgia State University, Atlanta, Georgia. July 2 4, 1997. a Jemilohun, P.F.; Ruiz-Ruano, A. and Villalta, F. 1993. Molecular cloning and Expression of a human immunogenic and stage-specific surface protein of Trypanos oma cruzi Trypomastigotes. Abstract. Annual General Meeting, American Society for Microbiology 1993, Atlanta, Georgia. a Villalta, F.; Lima, M.F.; Ruiz-Ruano, A.; Zhous, L. and Jemilohun, P.F. 1992 . A monoclonal antibody recognizes a Trypanosoma cruzi trypomastigote stage-spe
cific epitope on the trypomastigote 83 kDa required for parasite attachment to h ost cells. Federation of American Society for Experimental Biology. April 5-9, 1992. Anaheim, California. a C.W. Clark, J.B. Stukes, P.F. Jemilohun, A.C. Williams and E.R. Archibold 198 9. Characterization of plasmid DNA-membrane complexes and their roles in plasmid DNA maintenance. 89th Annual Meeting of the American Society for Microbiology. May 14-18. New Orleans, Louisiana. a E. Archibold, P. Jemilohun and C. Clark. 1988. Identification of membrane-a ssociated forms of R6K plasmid DNA: Role of membrane in the maintenance of plasm id. Annual Meeting of Southeastern and South Carolina Branches. American Socie ty for Microbiology. October 6-8. Jekyll Island, Georgia. a E. Archibold, P. Jemilohun, J. Stukes and C. Clark. 1988. Analysis of Membr ane Associated Fractions of plasmid DNA Molecules in gram-negative and gram posi tive bacteria. 8th Annual Meeting of the American Society for Microbiology. Ma y 8-13. Miami Beach, Florida. a P.F. Jemilohun and E. R. Archibold. 1987. Analysis of Membrane-bound fraction of plasmid-DNA in Bacteria. NIH Centennial MBRS-MARC Symposium. Washington, DC.
andida albicans and Torulopsis glabrata in the presence of gentamicina. Research Instrumentation and Computer Utilization Skills a Utilized RT-PCR, construction of cDNA library, RNA/DNA isolation and Western/s outhern/northern blot techniques. ELISA assay, adhesion assay, blot overlay assa ys. a Use bio-markers in monitoring and interpreting tissue changes a High Performance Liquid Chromatography (HPLC) a" For separation of biological and chemical samples for bioactivity purposes. Philip F. Jemilohun 2 a Microscopy (fluorescence and light) for identification/recognition of sub-cell ular parts a High speed centrifugations for separation/purification of biomolecules and org anelles. Statistical Analysis, excel, SPSS, Word Processing, Microsoft Word and Word Perf ect Visual Presentation, Paint, Power Point, PhotoShop Teaching Experience 2007 - 2009. Associate Professor. Albany State University, Department of Natural Sciences, Albany GA. Teaching assignment included Microbiology, Genetics and sp ecial topic in ecology (Microbial Ecology) at graduate level and Immunology, Gen eral Biology, Study Skill, Research/Independence study and Introductory Biology at undergraduate level. I participated in mentoring HBCU-UP/STEM students in res earch and also supervised senior research projects. The findings from some of th e student projects have been presented at many national conferences. 2002 - 2006. Associate Professor. Biology Department, University of Arkansas, P ine Bluff Arkansas. Teaching assignment included Cell Biology, Microbiology, Par asitology and General Biology. 2000 - 2002 Virginia State University, Petersburg. Virginia. I taught Microbiolo gy, Immunology, Virology, Cell Physiology, Molecular Biology and Introductory Bi ology courses. 1993 - 2000 Assistant/Associate Professor. Jackson State University, Jackson. Mi ssissippi. I taught Microbiology and Molecular Cell Biology at both graduate and undergraduate levels, Independent study and General Biology/Introductory Biolog y courses for biology majors and non-majors. I developed a Molecular Biology cou rse for undergraduate students. Faculty Research Mentor I supervised research projects of three undergraduate students (two on MARC, and one on ONR programs). I also supervised three master degree theses and I was a member of dissertation committee. I was also a faculty research mentor and an i nstructor for Bridges to the Baccalaureate Degree Program (BBDP), Jackson State University/Hinds Community College. Minority students from Hinds Community Colle ge performed research in my laboratory during academic year and summer. Their re search studies have been presented at various symposia such as at University of Memphis, Tennessee in summer 1996, Georgian State University, Atlanta, Georgia i n summer 1997 and University of Southern Alabama, Mobile, AL in summer 1999. Professional Affiliation and Awards American Society for Microbiology Beta Kappa Chi Scientific Honor Society Minority Biomedical Research Service (MBRS) award for doctoral program National Research Service Award for postdoctoral training 1990-1992 Research Grants 2008. Faculty Development Award for $10,000.
2003-2006. NIH/AREA grant: aFlagellar antigens from Trichomonas vaginalisa was f unded for $100,000. 1995. Faculty Research Award by Mississippi Urban Research Center (MURC) for $10 ,000 1994. Awarded aseed funda for $4,500. Professional Development Various courses, seminars, workshops and conferences supplement my extensive exp eriences in teaching, services, and research. a DNA micro-array workshop. Morehouse College Atlanta Ga. July 2005 a Quality Education for Minorities (QEM) Network/NSF proposal workshop. Janua ry 2005. a Served as a judge at many regional and state science fairs. a Attended Y2K Research/Teaching Symposium. Washington University, St. Louis M O. June 2000 Philip F. Jemilohun 3 Publications C Philip Jemilohun. Analysis of adherent and non-adherent Trichomonas vaginalis and identification of potential novel adhesins. Manuscript in preparation. a Philip Jemilohun and Tedra Thomas. Immunogenicity and adhesive property of fl agella from Trichomonas vaginalis. J. Parasitology. Manuscript in preparation. C Jemilohun, P.F. Garnett, T. and Burgess, D. E. 2005. Isolation, partial purifi cation, and immunogenicity of flagella from Tritrichomonas foetus. J. Eukaryotic Microbiology, 52:245-249. a Jemilohun, P.F. 1998. Isolation and Characterization of Flagella from Trichomo nas vaginalis. Parasitology Research. 84: 800-805. a Jemilohun, P. F.; Clark, C. W. and Archibold, E. R. 1996. Characterization of Bacterial Cell Membrane Attachment Sites of Plasmid R6K. Biochem. and Biophys. Res. Commun. 221: 186-192. a Jemilohun, P. 1991. Developmentally Regulated Surface Polypeptides of Invasi ve forms of Trypanosoma cruzi. Tropical Diseases. Meharry Medical College Train ing Program Newsletter. Abstracts & Poster Presentations a Jemilohun, P. F. Analysis of adherent and nonadherent Trichomonas vaginalis c ells and identification of potential novel adhesins. UAPB Faculty and Students R esearch Forum. March, 2006. a Jemilohun, P.F. Characterization of flagella from Tritrichomonas foetus. Abs tract D-141. 101 Annual General Meeting, American Society for Microbiology 2001, Orlando, Florida. May 2001. a Layfield, N., Luckett, J. and Jemilohun, PF. 1999. Flagellar Antigens of Trit richomonas foetus. National Minority Research Symposium. Phoenix. November 10-13 . a Hall, A., Wright, K. and Jemilohun, P.F. Characterization of flagella from T ritrichomonas foetus. National Minority Research Symposium. New York City. No vember 1998. a Barber, L.P. and Jemilohun, P.F. SDS-PAGE Analysis of flagella from Trichomo nas vaginalis and Tritrichomonas foetus. Mississippi Academy of Science Annual Meeting, 1998. a Mary Green, Chelsea Spann, Benjamin Torrey, Toshma Thomas-Carter and Philip J emilohun. SDS-PAGE Analysis of Flagella from Tritrichomonas foetus. Second Annua l Bridge Research Conference, Georgia State University, Atlanta, Georgia. July 2 4, 1997. a Jemilohun, P.F.; Ruiz-Ruano, A. and Villalta, F. 1993. Molecular cloning and Expression of a human immunogenic and stage-specific surface protein of Trypanos oma cruzi Trypomastigotes. Abstract. Annual General Meeting, American Society for Microbiology 1993, Atlanta, Georgia. a Villalta, F.; Lima, M.F.; Ruiz-Ruano, A.; Zhous, L. and Jemilohun, P.F. 1992 . A monoclonal antibody recognizes a Trypanosoma cruzi trypomastigote stage-spe
cific epitope on the trypomastigote 83 kDa required for parasite attachment to h ost cells. Federation of American Society for Experimental Biology. April 5-9, 1992. Anaheim, California. a C.W. Clark, J.B. Stukes, P.F. Jemilohun, A.C. Williams and E.R. Archibold 198 9. Characterization of plasmid DNA-membrane complexes and their roles in plasmid DNA maintenance. 89th Annual Meeting of the American Society for Microbiology. May 14-18. New Orleans, Louisiana. a E. Archibold, P. Jemilohun and C. Clark. 1988. Identification of membrane-a ssociated forms of R6K plasmid DNA: Role of membrane in the maintenance of plasm id. Annual Meeting of Southeastern and South Carolina Branches. American Socie ty for Microbiology. October 6-8. Jekyll Island, Georgia. a E. Archibold, P. Jemilohun, J. Stukes and C. Clark. 1988. Analysis of Membr ane Associated Fractions of plasmid DNA Molecules in gram-negative and gram posi tive bacteria. 8th Annual Meeting of the American Society for Microbiology. Ma y 8-13. Miami Beach, Florida. a P.F. Jemilohun and E. R. Archibold. 1987. Analysis of Membrane-bound fraction of plasmid-DNA in Bacteria. NIH Centennial MBRS-MARC Symposium. Washington, DC.
